Cell and Tissue Imaging Core

The Cell and Tissue Imaging Core enhances activities and accomplishments of ongoing research projects by providing histology services, access to a range of advanced microscopic imaging equipment and technical expertise. It provides essential resources for microscopic evaluation of ocular tissues and increase the innovative capabilities of participating investigators for conducting productive basic and translational vision research. The Core provides equipment and technical expertise for:

  1. Performing histology of ocular tissue specimens
  2. Enabling investigators to evaluate ocular tissues with the use of sophisticated light/confocal and electron microscopy and emerging imaging modalities
  3. Training investigators and their research staff on the utility and operation of cell and tissue imaging equipment
  4. Consultations on cell and tissue preparation and imaging for experimental design and appropriateness of techniques to achieve research goals

Core Faculty/Staff

Core Director: Sarah Hamm-Alvarez, PhD
Core Co-Director: David Hinton, MD

Core Staff:

  • Research Specialist: Charles Zamilpa (zamilpa@usc.edu)
  • Research Specialist: Xiaopeng Wang

Equipment

Laser Confocal and Live Cell Imaging

  • Zeiss Modular Laser Scanning Microscope – LSM 800 Airyscan, 3-Channel System
  • Zeiss Axio Observer 7 Imaging System
  • Zeiss AXIO Imager.A2 upright transmitted light and fluorescent microscope

Live Cell and Sample Preparation Areas

  • LABCONCO Biological Safety Cabinet
  • Wet Laboratory

Histology Laboratory

  • Microtomes (Leica RM2125RT)
  • Tissue Processor (Leica TP1020)
  • Embedding Station (Leica EG1150 H+C)
  • Microscope (Leica CM)
  • Cryostats (International equipment company Minotome, Reichert Histostat)
  • Ultracut R Microtome (Leica)
  • Dissecting Microscope MZ16 with DC camera and computer imaging station (Leica)

Electro Microscopy (available through the USC Core Center of Excellence in Nano Imaging)

  • Helios G4 PFIB UXe DualBeam FIB/SEM
  • JSM 7001F Field Emission Scanning Electron Microscope
  • Nova NanoSEM 450 Field Emission Scanning Electron Microscope
  • JEM-2100F Transmission Electron Microscope
  • FEI Talos F200C G2 Biological Transmission Electron Microscope

Optical Imaging (available through the USC Optical Imaging Facility)

  • Confocal Microscope: Leica SP-8X with Digital Light Sheet (DLS) (inverted configuration)
  • Confocal Microscope: Leica SP-8X with Multiphoton Laser (upright configuration)
  • Confocal Microcrope: Zeiss LSM 780 AxioObserver.Z1 (inverted configuration)
  • Confocal Microscope: Zeiss LSM 800: AxioObserver.M2 (upright configuration)
  • Widefield Microscope: Leica Thunder Computational Clearing (inverted configuration)
  • Wide-field Fluorescent Microscopes: Zeiss AxioImager.A1 (upright configuration)
  • Wide-field Fluorescent Microscopes: Zeiss AxioObserver.A1 and Axiovert 200 (inverted configuration)
  • Wide-field Fluorescent Microscope: Zeiss AxioZoom .v16
  • Slide Scanner: Zeiss AxioScan.z1

Translational Imaging Center (available through the Translational Imaging Center)

  • Two-Photon single plane illumination microscopy (SPIM) Light Sheet Imager
  • Hyperspectral Phasor Imaging (HPI)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)

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