Cell and Tissue Imaging Core

The Cell and Tissue Imaging Core enhances activities and accomplishments of ongoing research projects by providing histology services, access to a range of advanced microscopic imaging equipment and technical expertise. It provides essential resources for microscopic evaluation of ocular tissues and increase the innovative capabilities of participating investigators for conducting productive basic and translational vision research. The Core provides equipment and technical expertise for:

  1. Performing histology of ocular tissue specimens
  2. Enabling investigators to evaluate ocular tissues with the use of sophisticated light/confocal and electron microscopy and emerging imaging modalities
  3. Training investigators and their research staff on the utility and operation of cell and tissue imaging equipment
  4. Consultations on cell and tissue preparation and imaging for experimental design and appropriateness of techniques to achieve research goals

Core Faculty/Staff

Core Director: Sarah Hamm-Alvarez, PhD
Core Co-Director: David Hinton, MD

Core Staff:

  • Research Specialist: Maria Edman-Wolcott, PhD (edman@usc.edu)
  • Research Specialist: Xiaopeng Wang

Equipment

Laser Confocal and Live Cell Imaging

Electron Microscopy (available through the USC Norris Cell and Tissue Imaging Core)

  • JEOL JEM-2100 LaB6 Transmission Electron Microscope (TEM)
  • JEOL 1011 Transmission Electron Microscope
  • JEOL JSM-6390LV Scanning Electron Microscope (SEM)

Microscopy and Image Analysis (available through the USC Norris Cell and Tissue Imaging Core)

  • Aperio Digital ScanScope
  • RMC MTX Ultramicrotome

Live Cell and Sample Preparation Areas

  • LABCONCO Biological Safety Cabinet
  • Wet Laboratory

Histology Laboratory

  • Microtomes (Leica RM2125RT)
  • Tissue Processor (Leica TP1020)
  • Embedding Station (Leica EG1150 H+C)
  • Microscope (Leica CM)
  • Cryostats (International equipment company Minotome, Reichert Histostat)
  • Ultracut R Microtome (Leica)
  • Dissecting Microscope MZ16 with DC camera and computer imaging station (Leica)

Translational Imaging Center (available through the Translational Imaging Center)

  • Two-Photon single plane illumination microscopy (SPIM) Light Sheet Imager
  • Hyperspectral Phasor Imaging (HPI)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)

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